The porta patch is routinely provided with an axonmdc multiclamp amplifier or an epc 10 amplifier from heka together with a computer and the software packages. The epc 10 usb patch clamp amplifiers are succes sors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990123, and the well known epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped setup, which includes a patch. Current injection path via the injection capacitor. Although either two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro amplifiers are combined in a single housing. Winwcp is a data acquisition and analysis program for handling signals from wholecell electrophysiological experiments. Patch clamp amplifiers from single channels to large macroscopic recordings. This paper describes various user interfaces that have been implemented, including a highlevel programming interface, a user interface based on the postscript language, and. Heka elektronik single patch clamp amplifier epc 10 epc10 for. Increased current injection capability by a factor of 5. Electrophysiology systems blm planar lipid bilayer workstation.
Heka elektronik patch clamp amplifiers, potentiostats. Small molecules for early endosomespecific patch clamping. Want to know more about our quality products or need assistance in technical support. The epc 10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the well known. The novel program design of patch master is perfectly suited for the operation of multichannel stimulation as used for patch clamp amplifiers with multiple headstages e. The epc 10 usb patch clamp amplifier is available with either one epc 10 usb, two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro integrated amplifier modules. It helps in current clamp recordings, field potential recordings in ion channel and highspeed current clamp recording within the same head stage. Epc 9 the fully computer controlled patchclamp amplifier with builtin interface board. Click here to learn about the new features and bugfixes and download the new version. Continuing the tradition of providing the worlds best electrophysiology amplifiers, the epc 10 usb double, triple, and quadro patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. Digital control of filter settings, gain and compensation networks allows the admittance of the signal source at the amplifiers input to be determined based on the amplifiers own calibration. Nanion portapatch planar patch clamping automate scientific.
The elc03xs amplifier is the only commercially available amplifier that is not only designed for the loose patch clamp technique for recording in slice or in vivo preparations, but also for a wide range of other electrophysiological experiments. Epc 10 usb single patch clamp amplifier epc 10 usb double triple quadro patch clamp amplifiers data acquisition ced data acquisition and analysis ced power140 amplifier ced micro140 amplifier ced 1902 isolated pre amplifier in vitro measystems axion biosystems. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is a fully equipped recording setup. No selects the amplifier channel displayed on the panel when a multichannel amplifier is in use. Heka elektronik single patch clamp amplifier epc 10 epc10. We use igor software to do our analysis and make publication ready. Epc 10 usb heka patch clamp amplifiers warner instruments. How much it will cost on the average to obtain and set up. Used heka elektronik epc 10 single patch clamp amplifier unit. Epc 10 usb double triple quadro patch clamp amplifiers. A patch clamp amplifier and corresponding headstage are also included when purchasing patch clamp recording systems. Epc 10 usb single patch clamp amplifier bronjo medi.
It has neither frontpanel controls nor internal trim adjustments. The epc 7 represents roughly the seventh in the series of patch. For the screening of pharmaceutical compounds or the characterization of expressed channels, highthroughput patchclamp systems are being developed, where recordings are made from cells in each well of a 96 or 384well plate. Admittancebased measurement of membrane capacitance using. The advanced integration of the epc 10 usb with the interface and software minimizes total recording noise, eliminates compatibility problems and reduces additional. Sigworth department of cellular and molecular physiology, yale university school of medicine, 333 cedar street, new haven, ct 06510, usa received 30 march 1994. Epc 10 usb single patch clamp amplifiers, potentiostats. The patchclamp amplifier is a ubiquitous tool for characterizing ionchannel activity. A dll dynamic link library is available to allow access of the system from a custom written application. The epc 10 usb double, triple, and quadro patch clamp amplifiers are the optimal instruments for performing double or triple patch experiments. There are 16 additional digital outputs and 16 digital inputs available at the rear panel of the epc 10 usb. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped recording setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a full featured data acquisition and analysis system. There are many advantages to a fully digitallycontrolled patch clamp amplifier. Dear all, im looking to purchase a new patch clamp amplifier.
A headstage must be selected and included when purchasing a patch clamp amplifier. Used heka elektronik epc 10 single patch clamp amplifier. The letters epc stand for extracellular patch clamp, the method introduced by neher and sakmann 1976 for recording the currents in a small patch of membrane under voltage clamp conditions. Design of the epc9, a computercontrolled patchclamp. Apply the cfast fastcapacitance correction, wait for the seal to stabilize between 5 10 g. Extracellular, intracellular and patch clamp amplifier. Epc 10 usb heka patch clamp amplifiers pc505b patch clamp amplifier pc501a patch clamp amplifier. Currents were recorded using an epc10 patchclamp amplifier heka, lambrecht, germany and patchmaster acquisition software heka. The portfolio of amplifiers includes axopatch 200b for ultra lownoise singlechannel recordings, multiclamp 700b for wholecell voltage clamp and highspeed current clamp recordings. Patch clamp amplifiers, auto planer oocyte scitech. This amplifier enables an investigator to identify a single cell in a preparation by its characteristic spike train, then inject dye. The master software family with its members patchmaster, fitmaster, potmaster, pgmaster, chartmaster and epcmaster have been updated to version 2x90. Epc 10 usb patch clamp amplifier family electrophysiology models and features epc 10 usb single patch clamp amplifier the epc 10 is a complete data acquisition system, which can be used with hekas patchmaster or tida software. The patchserver is an addon tool for automating a manual patch clamp setup.
Epc 10 usb patch clamp amplifier family epc 10 usb double, triple, quadro patch clamp amplifier the epc 10 usb double, triple and quadro amplifiers are the optimal instruments for performing multiple patch cell experiments. Heka elektronik epc 10 usb hardware manual pdf download. The heka epc10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the wellknown epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. Epc 10, heka,patch clamp,singapore,amplifiers bronjo medi. Patch clamp recordings in the whole cell configuration, and single channel recordings in the cell attached configuration, can be performed with planar patch clamp chips. Im looking to purchase a new patch clamp amplifier. Heka elektronik epc 10 single patch clamp amplifier good working condition, was removed by us from a large pharma lab that concentrated on the electrophysiology studies.
For the most uptodate information on warner instruments news and technologies, signup for our newsletter. Each amplifier is controlled independently through heka patchmaster software. The epc 9 patch clamp amplifier is a digitally controlled analog device for recording currents in membrane patches and in small cells. Axon instruments appears to be the gold standard theyre the.
The epc 9 or epc 10 is the ideal patch clamp amplifier for capacitance measurements because all the relevant parameters are under its control. Unlike other patch clamp amplifiers, the model 2400 has a voltage follower in the probe. An integrated four pole low pass bessel filter provides flexible signal conditioning. The epc 800 usb can be controlled with any data acquisition interface and can be supported, not only by heka software, but. Pdf design of the epc9, a computercontrolled patchclamp. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or patchmaster next software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. Amplifier for voltage clamping using patch electrodes on. Heka is now shipping epc 10 usb patch clamp amplifiers of hardware revision t. Filter 2 usable for current or voltage signal more. Heka is now shipping epc 10 usb patch clamp amplifiers.
The downscaling is necessary to avoid saturation of the lockin amplifier because the output range of the epc7 is 10 v, whereas the maximum input range of the lockin amplifier is only. Elsevier journal of neuroscience methods 56 1995 195202 design of the epc 9, a computercontrolled patchclamp amplifier. Heka epc10 usb patch clamp amplifier online at digitimer uk. The unique feature of this virtual instrument is that it is thoroughly integrated with a computercontrolled patch clamp amplifier epc 9 to allow estimation of equivalent circuit parameters based upon calibrated admittance. Although either two epc 10 usb double, three epc 10 usb triple or four epc 10 usb quadro amplifiers are.
Three major advantages em ployed in the design of the epc 10 usb are. The amplifier gain, compensation and currentvoltage clamp mode of tecella patch clamp amplifiers can be set from this control panel. Although two, three or four amplifiers are combined in a single housing, along with. The system allows a highspeed threshold detection epc 10 usb patch clamp amplifier sends out a voltage signal that helps the user to determine when the tip makes contact with the cell membrane. Current clamp recordings with the epc 10 patch clamp. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or tida software is equivalent to a fully equipped setup, which includes a. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster or tida software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. Cc circuitry of the epc 10 amplifier current injection path via the feedback resistor of the patch clamp headstage. This allows this amplifier to be a true fast current clamp amplifier with no instability. The epc10 is well suited for both voltage and current clamp. Pdf design of the epc9, a computercontrolled patch. Heka is pleased to release the newest family of fully computer controlled patch clamp amplifiers. Imaging extension for patchmaster, potmaster and chartmaster. Fast and slow capacitive transients were cancelled by the compensation circuit of the epc 10 amplifier.
Novel aspects of the circuit design of this instrument are discussed, with. If a dongle is not attached to the computer then the programs will run in demo mode. The output of either patch clamp amplifier is scaled down by a factor of 10 before input to the lockin amplifier by a 10 k. Patchclamp amplifiers on a chip pubmed central pmc.
Heka is very proud to release the epc 10 usb the newest member of the epc 10 family of fully. The unique feature of this virtual instrument is that it is thoroughly integrated with a computercontrolled patch clamp amplifier epc 9 to allow estimation of equivalent circuit parameters based upon calibrated admittance measurements. The epc 9 computercontrolled amplifier has no frontpanel controls. The epc 10 usb patch clamp amplifiers are successors of the revolutionary epc 9 patch clamp amplifiers, which were first introduced in 1990, and the well known epc 10 patch clamp amplifiers, which replaced the epc 9s in 2002. A software lockin amplifier slia was developed to allow hightimeresolution measurement of membrane capacitance as a singlecell assay of exocytosis. Apply the cfast fastcapacitance correction, wait for the seal to stabilize between 510 g. Epc 10 usb heka patch clamp amplifiers pc505b patch clamp amplifier pc501a patch clamp amplifier need support.
It is a logical successor to the classical epc 7, retaining all of its features but adding a number of capabilities. This amplifier is truly a unique hybrid patch clamp amplifier with its design and featureset primarily based upon the manually controlled epc 8. Apparatus suitable for conducting a method according to any of claims 1 to 8, which comprises the probe, means for measuring andor controlling the distance of the probe from a surface, means for bringing the probe into contact with the surface, and a patch clamp amplifier. Axon instruments patchclamp amplifiers molecular devices. Heka elektronik download the latest software release. Q series electrode holders str, port, ag wire sizes 1. The epc 10 usb patch clamp amplifier, combined with a computer and patchmaster software is equivalent to a fully equipped setup, which includes a patch clamp amplifier, a digital storage oscilloscope, a variable analog filter, a sophisticated pulse generator, and a fully featured data acquisition and analysis system. Axopatch patch clamp amplifier is the premier microelectrode amplifier for ultralow noise patch clamp recordings.
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